Amino Acid Mixture, Complete from Promega

Product Specs
ItemAmino Acid Mixture, Complete
CompanyPromega
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberL4461
Quantity175microl
Molecular Weight~74kDa

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Promega

2800 Woods Hollow Rd.

Madison, Wisconsin

United States

Phone: 800-356-9526
Fax: 800-356-1970

Company Profile

Website: http://www.promega.com

(1) Nitric oxide-induced ribosome collision activates ribosomal surveillance mechanismsCell Death & DiseaseJuly 26, 2023Laura Ryder, Frederic Schrøder Arendrup, José Francisco Martínez, Goda Snieckute, Chiara Pecorari, Riyaz Ahmad Shah, Anders H. Lund, Melanie Blasius, Simon Bekker-Jensen(Sigma Aldrich, #10127566001), 10 µM spermidine (Sigma Aldrich, # S0266), 10 µM amino acids (Promega, # L4461), 65 mM KAc, 0.75 mM MgAc2 and murine RNase inhibitor A dual mRNA construct with a Renilla luciferase

(2) Human-rabbit Hybrid Translation System to Explore the Function of Modified RibosomesBIO-PROTOCOLJuly 5, 2023Eriko Matsuura-Suzuki, Hirotaka Toh, Shintaro Iwasakicatalog number: 16229-95, stored at room temperature)Amino acid mixtures (Promega, catalog number: L4461, stored at -80 °C)Recombinant RNase inhibitor (TaKaRa, catalog number: 2313A, stored at -20 °C)Dual-Luciferase Reporter Assay System

(3) Production of human translation-competent lysates using dual centrifugationRNA BiologyDecember 31, 2022Lukas-Adrian Gurzeler, Jana Ziegelmüller, Oliver Mühlemann, Evangelos D. Karousis0.68 mM MgCl2, 54.05 mM KCl, 0.05 mM amino acid mixture, unless otherwise stated (Promega, Cat. No. L4461), 13.51 mM Creatine Phosphate, 229.73 ng/ml Creatine kinase and 1x protease inhibitor cocktail (Bimake,

(4) Functional characterization of 5′ UTR cis-acting sequence elements that modulate translational efficiency in Plasmodium falciparum and humansMalaria JournalDecember 1, 2022Valentina E. Garcia, Rebekah Dial, Joseph L. DeRisi384-well plates. A master mix of 3.5 µL lysate with 0.5 µL 100 μm complete amino acid mix (Promega L4461) and 1uL 10x translation buffer (20 mM HEPES pH 8, 75 mM KoAc, 2 mM DTT, 5 mM ATP, 1 mM GTP 200 mM creatine

(5) PABP/purine-rich motif as an initiation module for cap-independent translation in pattern-triggered immunityCellAugust 18, 2022Wang J, Zhang X, Greene GH, Xu G, Dong X.#TER51020 Phos-tag (TM) Acrylamide,FUJIFILM Wako,Cat. #304–93521 "Amino Acid Mixture, Complete",Promega,Cat. #L4461 m7G(5′)ppp(5′)G RNA Cap Structure Analog,NEB,Cat. #S1404S SUPERase•In RNase Inhibitor,Invitrogen,Cat.

(6) NusG-mediated Coupling of Transcription and Translation Enhances Gene Expression by Suppressing RNA Polymerase BacktrackingJournal of Molecular BiologyJanuary 1, 2022Elizabeth J. Bailey, Max E. Gottesman, Ruben L. Gonzalezthe reaction; 5 μL of a solution that is 1 mM in each of the 20 essential amino acids (Promega, No. L4461); 20 μl of Promega S30 Premix without Amino Acids (No. L512A-C); vS30 μL of S30 cell extract, where

(7) Development, validation, and application of the ribosome separation and reconstitution system for protein translation in vitroRNADecember 1, 2021Brandon M. Trainor, Dimitri G. Pestov, Natalia Shcherbiksolution of 20 essential amino acids, complete and minus Methionine and Cysteine, were from Promega (cat# L4461 and L5511, respectively); Ambion mMESSAGE mMACHINE T7 Transcription kit was purchased from Thermo Fisher Scientific

(8) Small Extracellular Vesicles Propagate the Inflammatory Response After TraumaAdvanced ScienceDecember 1, 2021Tanja Seibold et al.reticulocyte lysate system (Promega, #L4960) and non‐labeled complete amino acid mixture (Promega, #L4461) according to the manufacturer's description. After the translation reaction, samples were supplemented

(9) 5′ Half of specific tRNAs feeds back to promote corresponding tRNA gene transcription in vertebrate embryosScience AdvancesNovember 19, 2021Luxi Chen et al.reaction was assembled as follows: 9.9 μl of RRL, 0.3 μl of 1 mM complete amino acid mixture (Promega, L4461), 0.42 μl of 3 M KCl, 0.39 μl of RNase inhibitor, 1 μl of GFP mRNA (500 ng/μl), 0.5 μl of purified NLS-2xFlag-mCherry-HisTag

(10) The eIF2 kinase GCN2 directs keratinocyte collective cell migration during wound healing via coordination of reactive oxygen species and amino acidsThe Journal of biological chemistryNovember 1, 2021Miles RR, Amin PH, Diaz MB, Misra J, Aukerman E, Das A, Ghosh N, Guith T, Knierman MD, Roy S, et al.of 1000 V/cm. A calibration curve was prepared using a sample containing all 20 amino acids (Promega L4461). The amino acid standard was diluted 1:100 to make 10 μM standard in BGE metabolite diluent provided

(11) The molecular basis of coupling between poly(A)-tail length and translational efficiencyeLifeJuly 2, 2021Xiang K, Bartel DP.reticulocyte lysate or oocyte extract in total volume of 20 µl containing 10 µM amino acid mix (Promega, L4461), 0.5 units SUPERase•In, 10 mM creatine phosphate (Roche 10621714001), 200 µg/ml creatine kinase (Roche,

(12) Selective inhibition of human translation termination by a drug-like compoundNature CommunicationsDecember 1, 2020Wenfei Li, Stacey Tsai-Lan Chang, Fred. R. Ward, Jamie H. D. Catespermidine, 0.6 mM putrescine, 2 mM TCEP (tris(2-carboxyethyl)phosphine), 10 μM amino acid mixture (Promega, L4461), 1 U μL−1 murine RNase inhibitor (NEB, M0314L), 600 ng of mRNA, and 50 μM PF846 in 1% DMSO as control.

(13) A broad-spectrum antiviral molecule, QL47, selectively inhibits eukaryotic translationJournal of Biological ChemistryFebruary 1, 2020Mélissanne de Wispelaere et al.phosphokinase (Sigma, 10127566001), 2 mm DTT, 2.5 mm Mg(OAc)2, 100 mm K(OAc), 8 μm amino acids (Promega, L4461), 255 μm spermidine, and 1000 units/ml murine RNase inhibitor. Small molecules were added to the reaction

(14) A Yeast System for Discovering Optogenetic Inhibitors of Eukaryotic Translation InitiationACS Synthetic BiologyApril 19, 2019Huixin Lu, Mostafizur Mazumder, Anna S. I. Jaikaran, Anil Kumar, Eric K. Leis, Xiuling Xu, Michael Altmann, Alan Cochrane, G. Andrew Woolley0.6 mM GTP, 150 mM creatine phosphate (Roche SKU 10621714001), 0.24 mM 20 amino acid mix (Promega SKU L4461), and 10.2 mM DTT. In the dark, the pre-mix was split into 4 equal aliquots of 24 μL . Each aliquot

(15) Multiple prebiotic metals mediate translationProceedings of the National Academy of SciencesNovember 27, 2018Marcus S. Bray, Timothy K. Lenz, Jay William Haynes, Jessica C. Bowman, Anton S. Petrov, Amit R. Reddi, Nicholas V. Hud, Loren Dean Williams, Jennifer B. Glassfrom the PURExpress Δ Ribosome Kit (E3313S; New England Biolabs), 0.1 mM amino acid mix (catalog # L4461; Promega), Bray et al. and 0.2 mM tRNAs from E. coli MRE 600 (product # TRNAMRE-RO; SigmaAldrich).

(16) Highly efficient in vitro translation of authentic affinity-purified messenger ribonucleoprotein complexesRNAJuly 1, 2018Sarah E. Fritz, Nazmul Haque, J. Robert HoggRoche), 0.8 mM ATP (P0756S; New England BioLabs), 0.1 mM spermidine, 0.1 mM complete amino acid mix (L4461; Promega), 135 mM potassium acetate (for purified mRNPs) or 50 mM potassium acetate (for IVT mRNA and

(17) Generation of ribosome imprinted polymers for sensitive detection of translational responsesScientific reportsJuly 26, 2017King HA, El-Sharif HF, Matia-González AM, Iadevaia V, Fowotade A, Reddy SM, Gerber AP.phosphate (Merck Millipore #2380), 1 mg/ml yeast tRNA (Sigma #R4752), 2 µl amino acids mix (Promega #L4461), 0.5 µl RNasin (Promega #N2615), 10 µl reticulocyte lysate (Promega #L4151), and 0.5 µM CHX (Sigma

(18) Surface-enhanced Raman spectroscopy competitive binding biosensor development utilizing surface modification of silver nanocubes and a citrulline aptamerJournal of Biomedical OpticsJuly 21, 2017Brian M. Walton, George W. Jackson, Nicolaas Deutz, Gerard Coteodd-numbered rounds, the library was first exposed to a cocktail of the 20 essential amino acids (Promega, catalog #L4461) followed by citrulline elution in phosphate-buffered saline (PBS) buffer, pH 7.4, 1 mM MgCl2 , 0.05%

(19) Condensin II is anchored by TFIIIC and H3K4me3 in the mammalian genome and supports the expression of active dense gene clustersScience AdvancesJune 21, 2017Kobe C. Yuen, Brian D. Slaughter, Jennifer L. Gertonmixed with 2 μl of TnT Reaction Buffer, 1 μl of TnT RNA Polymerase, 2 μl of Complete Amino Acid Mix (L4461, Promega), 1 μl of RNasin Ribonuclease Inhibitor (N2511, Promega), 2 μl of DNA plasmid, and 17 μl of

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